Timergazina I.F.

ins@vnigri.ru




Graduated from Chelyabinsk State University, Department of Biology, specialization medical microbiology (2011).
Engineer of the complex laboratory of physical and chemical methods of analysis of hydrocarbons, VNIGRI (since 2011).
Research interests: soil microbiology, ecology.
Raw hydrocarbon geoecology
Section editor – PhD in geology and mineralogy Rogozina E.A.
Article # 19_2014 submitted on 03/11/2014 displayed on website on 04/21/2014
16 p.
pdf Cleaning oil-contaminated soil by Psedomonas bacteria - basis of biological products NAFTOKS 12-R and NAFTOKS 48-U
The comparative characteristics of two newly created biologics NAFTOKS 12-R (Pseudomonas aeruginosa) and NAFTOKS 48-U (Pseudomonas citronelollis) on the effectiveness of cleaning soil from diesel fuel and oil in the laboratory are presented. For 7 months of experiments diesel fuel oxidized by 85.5-85.8% of initial content in the soil (? 2% wt.). Oil fractions in its content oxidized by 91.6 and 92.3%, benzene resins - 41.0 and 27.7%, alcohol-benzene resins - 39.8 and 37.5%, asphaltenes - 44.3 and 23.8% of the initial content. Methane-naphthenic hydrocarbons decreased by 92.0 and 92.3%, aromatic hydrocarbons - by 84.3-100% and 89.3-100% of the initial content, respectively, by adding NAFTOKS 12-R and NAFTOKS 48-U biologics in oil-contaminated soil. Biooxidation of oil contamination accompanied by formation of intermediate oxidation products. The fundamental principles of innovative technology of cleaning oil-contaminated soils using NAFTOKS biologics are discussed.

Keywords: oil-contaminated soil, bioremediation, NAFTOKS biologics, cleaning efficiency of soil, soil treatment technology.
article citation Rogozina E.A., Timergazina I.F., Morgunov P.A. Ochistka neftezagrjaznennyh pochv bakterijami roda Pseudomonas - osnovoj biopreparatov NAFTOKS 12-R i NAFTOKS 48-U [Cleaning oil-contaminated soil by Psedomonas bacteria - basis of biological products NAFTOKS 12-R and NAFTOKS 48-U]. Neftegazovaya Geologiya. Teoriya I Praktika, 2014, vol. 9, no. 2, available at: http://www.ngtp.ru/rub/7/19_2014.pdf
References
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   Biopreparaty serii NAFTOKS dlya ochistki pochvennykh i vodnykh ekosistem ot zagryazneniy [NAFTOKS biologicals series for cleaning soil and aquatic ecosystems from contaminations]. E.A. Rogozina, A.V. Khotyanovich, R.A. Arkhangel'skaya et al. Doklady Pervoy Vserossiyskoy konferentsii 'Poiski nefti, neftyanaya industriya i okhrana okruzhayushchey sredy'. Saint Petersburg, 1995, p. 137-144.
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   Pozdeev O.K. Meditsinskaya mikrobiologiya [Medical microbiology]. Moscow: GEOTAR-MED, 2001, 765 p.
   Preobrazovanie neftey mikroorganizmami [Oil converting using microorganisms]. Editors B.G. Khotimskiy and A.I. Akopian. Trudy VNIGRI. Leningrad, 1970, 281 p.
   Rogozina E.A. Geokhimicheskie izmeneniya v sostave neftey pri biodegradatsii [Geochemical changes in the composition of oils during biodegradation]. Razvedka i okhrana nedr, 2010, no. 4, p. 63-68.
   Rogozina E.A., Andreeva O.A., Zharkova S.I., Martynova D.A., Orlova N.A. Sravnitel'naya kharakteristika otechestvennykh biopreparatov, predlagaemykh dlya ochistki pochv i gruntov ot zagryazneniya neft'yu i nefteproduktami [Comparative characteristics of domestic biologics proposed for cleaning of soils from oil and oil products contamination]. Neftegazovaya geologiya. Teoriya i praktika, 2010, vol. 5, no. 3, available at: http://www.ngtp.ru/rub/7/37_2010.pdf
   Rozanova E.P., Kuznetsov S.I. Mikroflora neftyanykh mestorozhdeniy [Microflora of oil fields]. Moscow: Nauka, 1974, 197 p.
   Skryabin G.K., Golovleva L.A. Ispol'zovanie mikroorganizmov v organicheskom sinteze [Use of microorganisms in organic synthesis]. Moscow: Nauka, 1976, 332 p.
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Raw hydrocarbon geoecology
Section editor – PhD in geology and mineralogy Rogozina E.A.
Article # 16_2013 submitted on 04/30/2013 displayed on website on 05/25/2013
14 p.
pdf  Naftoks biological products for clean up oil-contaminated soil
A new series of biologics of Naftoks brand was created on the basis of live active strains of hydrocarbon-oxidizing microorganisms to clean up oil-contaminated soil. Biological products have been laboratory tested in terms of efficiency of utilization of soil contaminated with diesel fuel and oils. The high degree of utilization of oil pollution in general (up to 87.4% of the initial content) and for specific fractions in its content (up to 95.6-100%) is shown. Moreover, the ability of created biological product to utilize specific fractions of petroleum contaminants is determined, which enable to produce biologics of target purpose. The implementation of research results into domestic oil refineries works depends on the interest and volitional decisions of the organizations involved in protection of natural ecosystems from oil pollution.

Key words: soil, oil pollution, clean up, biological products, hydrocarbon-oxidizing microorganisms, efficiency, balance calculations of cleaning process.
article citation Rogozina E.A., Morgunov P.A., Timergazina I.F., Shapiro A.I. Biopreparaty serii NAFTOKS dlya ochistki pochvy ot neftyanogo zagryazneniya [Naftoks biological products for clean up oil-contaminated soil]. Neftegazovaya Geologiya. Teoriya I Praktika, 2013, vol. 8, no. 2, available: http://www.ngtp.ru/rub/7/16_2013.pdf
DOI https://doi.org/10.17353/2070-5379/16_2013
References

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   Rogozina E.A., Khotyanovich A.V. Biorekreatsiya neftezagryaznennykh pochvennykh i vodnykh ekosistem [Bio-recreation of oil-contaminated soil and water ecosystems]. Doklady Pervoy Vserossiyskoy konferentsii «Poiski nefti, neftyanaya industriya i okhrana okruzhayushchey sredy. Saint Petersburg, 1995, p. 131-136.
   Rogozina E.A., Khotyanovich A.V., Arkhangel'skaya R.A., Zharkova S.I., Ipatov Yu.I., Kostyunicheva E.V., Orlova N.A., Svechina R.M., Sergeenok L.I., Shapiro A.I. Biopreparaty serii NAFTOKS dlya ochitki pochvennykh i vodnykh ekosistem ot neftezagryazneniy [Biologies series Naftoks for cleaning soil and water ecosystems from oil pollution]. Doklady Pervoy Vserossiyskoy konferentsii «Poiski nefti, neftyanaya industriya i okhrana okruzhayushchey sredy». Saint Petersburg, 1995, p. 137-144.
   Rogozina E.A., Morgunov P.A., Perekhodova L.S., Timergazina I.F. Metodicheskie aspekty laboratornogo issledovaniya effektivnosti utilizatsii neftyanogo zagryazneniya pochv biopreparatami [Methodological aspects of laboratory studies of the effectiveness of utilization of oil-contaminated soil using biological products]. Georesursy, 2012, no. 6 (48), p. 13-15.
   Rogozina E.A., Svechina R.M., Shapiro A.I., Perekhodova L.S., Timergazina I.F. Metodicheskie aspekty issledovaniya aktivnosti utilizatsii nefti i nefteproduktov shtammami uglevodorodokislyayushchikh mikroorganizmov [Investigation of utilizing oil and oil products by strains of hydrocarbon-oxidizing microorganisms - methodological aspects]. Neftyanaya geologiya. Teoriya i praktika, 2001, vol. 6, no. 4, available at: http://www.ngtp.ru/rub/7/43_2011.pdf



Raw hydrocarbon geoecology
Section editor – PhD in geology and mineralogy Rogozina E.A.
Article # 16_2012
28 p.
pdf  Biological oxidation of oil and petroleum products using hydrocarbon-oxidizing microorganisms
An analysis of published data concerning biological oxidation of various classes of hydrocarbons and petroleum products using hydrocarbon-oxidizing microorganisms has been carried out. The different mechanisms of hydrocarbon bio-oxidation have been discussed. The biogenic oxidation of oils of different chemical composition has been studied.
Key words: hydrocarbons, oxidation mechanism, hydrocarbon-oxidizing microorganisms, oil, petroleum products.
article citation Timergazina I.F., Perekhodova L.S. K probleme biologicheskogo okisleniya nefti i nefteproduktov uglevodorodokislyayushchimi mikroorganizmami [Biological oxidation of oil and petroleum products using hydrocarbon-oxidizing microorganisms]. Neftegazovaya Geologiya. Teoriya I Praktika, 2012, vol. 7, no. 1, available: http://www.ngtp.ru/rub/7/16_2012.pdf
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Raw hydrocarbon geoecology
Section editor – PhD in geology and mineralogy Rogozina E.A.
Article # 43_2011
9 p.
pdf  Investigation of utilizing oil and oil products by strains of hydrocarbon-oxidizing microorganisms - methodological aspects
The complex of laboratory experiments on the activity of utilizing contaminant oil by strains of hydrocarbon-oxidizing microorganisms developed in VNIGRI is analyzed. Particular attention is given to a detailed chemical bitumen analysis of initial and bio-oxidized samples, allowing investigating of utilization of contaminant oil and its fractions (oils, neutral and acidic resins, asphaltenes, saturated and aromatic hydrocarbons). Use of integrated chemical bitumen analysis in extraction contributes to objectiveness in evaluation the effectiveness of biological products for utilizing oil pollution of soil.
Key words: oil, oil pollution, hydrocarbons, petroleum products, strains of hydrocarbon-oxidizing microorganisms, activity, bio-oxidation, chemical bitumen analysis.
article citation Rogozina E.A., Svechina R.M., Shapiro A.I., Perekhodova L.S., Timergazina I.F. Investigation of utilizing oil and oil products by strains of hydrocarbon-oxidizing microorganisms - methodological aspects // Neftegazovaya Geologiya. Teoriya I Praktika. – 2011. - V.6. - #4.- http://www.ngtp.ru/rub/7/43_2011.pdf